Course detail

Advanced microscopic techniques in biology

FEKT-DBT3Acad. year: 2019/2020

Introduction to microscopy techniques and their use in biology. Fluorescence microscopy, dyes, their characterization and use. Histochemical, immunohistochemical and immunofluorescence. Microscopic methods for detection of cell organelles ( nucleus, nucleolus, endoplasmic reticulum, Golgi apparatus, mitochondria, cytoskeleton, lysosomes, vacuoles and other membrane structures). Microscopic detection cell function and apoptosis (programmed cell death). Detection ions, reactive oxygen and nitrogen. Nanoparticles and their use in microscopic techniques.

Learning outcomes of the course unit

Not applicable.


Not applicable.


Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Časopis Microscopy Research and Technique (Wiley)
Časopis Journal of Microscopy
Hoppert, M. Microscopic Techniques in Biotechnology, Wiley, 2001

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Not applicable.

Language of instruction


Work placements

Not applicable.

Course curriculum

1. Introduction to microscopic techniques. Light, fluorescence, confocal and electron microscopy.
2. Methods for the preparation of biological materials for various microscopic techniques and their specifics.
3. Fluorescence dyes used in microscopic techniques. Fluorescent dyes. Characterization, classification.
4. Histochemical, immunohistochemical and immunofluorescence techniques. Principles and applications.
5. Cell organelles, their structure and function I - using microscopic techniques.
6. Cell organelles, their structure and function II - using microscopic techniques.
7. Cell cycle and apoptosis. Microscopic detection of apoptosis (programmed cell death) as a complex process.
8. Microscopic detection of bioactive molecules (amines, thiols, carboxylic acids, proteins, etc.).
9. Microscopic detection of reactive oxygen and nitrogen.
10. Microscopic ion detection.
11. In situ hybridization.
12. Nanoparticles and their use in microscopic techniques


The aim of the course is to provide an overview of the application of individual microscopic techniques in cell biology with respect to cell structure and function .

Classification of course in study plans

  • Programme BTB-PP Doctoral

    branch PP-BTB , 1. year of study, winter semester, 4 credits, optional specialized

  • Programme BTB-PK Doctoral

    branch PK-BTB , 1. year of study, winter semester, 4 credits, optional specialized

Type of course unit



39 hours, optionally

Teacher / Lecturer