Czech

4

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1. Introduction to microscopic techniques. Light, fluorescence, confocal and electron microscopy.
2. Methods for the preparation of biological materials for various microscopic techniques and their specifics.
3. Fluorescence dyes used in microscopic techniques. Fluorescent dyes. Characterization, classification.
4. Histochemical, immunohistochemical and immunofluorescence techniques. Principles and applications.
5. Cell organelles, their structure and function I - using microscopic techniques.
6. Cell organelles, their structure and function II - using microscopic techniques.
7. Cell cycle and apoptosis. Microscopic detection of apoptosis (programmed cell death) as a complex process.
8. Microscopic detection of bioactive molecules (amines, thiols, carboxylic acids, proteins, etc.).
9. Microscopic detection of reactive oxygen and nitrogen.
10. Microscopic ion detection.
11. In situ hybridization.
12. Nanoparticles and their use in microscopic techniques

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The aim of the course is to provide an overview of the application of individual microscopic techniques in cell biology with respect to cell structure and function .

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Časopis Microscopy Research and Technique (Wiley)
Časopis Journal of Microscopy
Hoppert, M. Microscopic Techniques in Biotechnology, Wiley, 2001

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39 hours, optionally