Publication detail

DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology

UMAIR, M. SEDLÁŘ, K.

Original Title

DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology

Type

article in a collection out of WoS and Scopus

Language

English

Original Abstract

DNA methylation plays a crucial role in epigenetic modification which regulates gene expression and genome stability. Application of Pacific Biosciences sequencing technology (PacBio) and Oxford Nanopore sequencing technology (ONT) has shown promising approaches for detecting single base modification for DNA methylation. In this article, we analysed the base modification detection on ONT sequences and then compared it with PacBio analysed data. We found that both Nanopore and PacBio methods were effective but showed variations in their result. We analyzed the methylation pattern in Clostridium beijerinckii NRRL B-598 and found that the ONT data in combination with tombo tool can help detect modification at low coverage sites while avoiding false reading at these areas. Our study provide insight into these two approaches of DNA methylation detection, which will help in guiding the choices of appropriate method for specific research purposes.

Keywords

DNA Methylation; Clostridium beijerinckii; ONT; PacBio; 6mA

Authors

UMAIR, M.; SEDLÁŘ, K.

Released

28. 4. 2023

ISBN

978-80-214-6153-6

Book

PROCEEDINGS I OF THE 29TH STUDENT EEICT 2023

Pages from

275

Pages to

278

Pages count

4

URL

https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_1.pdf

BibTex

@inproceedings{BUT187062,
  author="Mohammad {Umair} and Karel {Sedlář}",
  title="DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology",
  booktitle="PROCEEDINGS I OF THE 29TH STUDENT EEICT 2023",
  year="2023",
  pages="275--278",
  isbn="978-80-214-6153-6",
  url="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_1.pdf"
}