Publication detail

The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions

NOVÁČKOVÁ, I. HRABALOVÁ, V. SLANINOVÁ, E. SEDLÁČEK, P. SAMEK, O. KOLLER, M. KRZYZANEK, V. HRUBANOVÁ, K. MRÁZOVÁ, K. NEBESÁŘOVÁ, J. OBRUČA, S.

Original Title

The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions

Type

journal article in Web of Science

Language

English

Original Abstract

Polyhydroxyalkanoates (PHA) are abundant microbial polyesters accumulated in the form of intracellular granules by numerous prokaryotes primarily as storage of carbon and energy. Apart from their storage function, the presence of PHA also enhances the robustness of the microbial cells against various stressors. In this work, we investigated the role of PHA in Cupriavidus necator, a model organism concerning PHA metabolism, for adaptation to osmotic pressure and copper ions. In long-term laboratory evolution experiments, the bacterial culture was cultivated in presence of elevated doses of sodium chloride or copper ions (incubations lasted 78 passages for Cu2+ and 68 passages for NaCl) and the evolved strains were compared with the wild-type strain in terms of growth and PHA production capacity, cell morphology (investigated by various electron microscopy techniques), activities of selected enzymes involved in PHA metabolism and other crucial metabolic pathways, the chemical composition of bacterial biomass (determined by infrared and Raman spectroscopy) and also considering robustness against various stressors. The results confirmed the important role of PHA metabolism for adaptation to both tested stressors.

Keywords

Adaptation; Cupriavidus necator; Heavy metals; Osmotic stress; Polyhydroxyalkanoates; Stress conditions

Authors

NOVÁČKOVÁ, I.; HRABALOVÁ, V.; SLANINOVÁ, E.; SEDLÁČEK, P.; SAMEK, O.; KOLLER, M.; KRZYZANEK, V.; HRUBANOVÁ, K.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.

Released

1. 5. 2022

Publisher

Elsevier

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

206

Number

5

State

Kingdom of the Netherlands

Pages from

977

Pages to

189

Pages count

13

URL

https://www.sciencedirect.com/science/article/pii/S0141813022005712?via%3Dihub

BibTex

@article{BUT178523,
  author="Ivana {Nováčková} and Vendula {Hrabalová} and Eva {Slaninová} and Petr {Sedláček} and Ota {Samek} and Martin {Koller} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Kateřina {Mrázová} and Jana {Nebesářová} and Stanislav {Obruča}",
  title="The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2022",
  volume="206",
  number="5",
  pages="977--189",
  doi="10.1016/j.ijbiomac.2022.03.102",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S0141813022005712?via%3Dihub"
}