Publication detail

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

PEKÁRKOVÁ, J. GABLECH, I. FIALOVÁ, T. BÍLEK, O. FOHLEROVÁ, Z.

Original Title

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

Type

journal article in Web of Science

Language

English

Original Abstract

Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.

Keywords

parylene-C; micropillars; selenium nanoparticles; biocompatibility; antimicrobial

Authors

PEKÁRKOVÁ, J.; GABLECH, I.; FIALOVÁ, T.; BÍLEK, O.; FOHLEROVÁ, Z.

Released

3. 12. 2021

Publisher

FRONTIERS MEDIA SA

Location

LAUSANNE

ISBN

2296-4185

Periodical

Frontiers in Bioengineering and Biotechnology

Year of study

9

Number

1

State

Swiss Confederation

Pages from

1

Pages to

11

Pages count

11

URL

https://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/full

Full text in the Digital Library

http://hdl.handle.net/11012/203324

BibTex

@article{BUT175415,
  author="Jana {Pekárková} and Imrich {Gablech} and Tatiana {Fialová} and Ondřej {Bílek} and Zdenka {Fohlerová}",
  title="Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability",
  journal="Frontiers in Bioengineering and Biotechnology",
  year="2021",
  volume="9",
  number="1",
  pages="1--11",
  doi="10.3389/fbioe.2021.782799",
  issn="2296-4185",
  url="https://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/full"
}