Double resin casting micro computed tomography (DUCT) of biliary and vascular system

Double resin casting micro computed tomography (DUCT) of biliary and vascular system

Abstract

Malformation of lumenized structures, such as the intrahepatic circulatory or biliary systems, is a hallmark of several liver disorders, from vascular hepatic disease to Alagille syndrome. Liver architecture in mice has typically been studied using 2D sections and, more recently, with thicker tissue sections and whole mount immunofluorescence, neither of which resolves 3D architecture adequately. Importantly, the vascular and biliary systems are inter-dependent, and we therefore aimed to develop a robust method to image, digitalize and quantify 3D architecture of the biliary and vascular systems in tandem.

Malformation of lumenized structures, such as the intrahepatic circulatory or biliary systems, is a hallmark of several liver disorders, from vascular hepatic disease to Alagille syndrome. Liver architecture in mice has typically been studied using 2D sections and, more recently, with thicker tissue sections and whole mount immunofluorescence, neither of which resolves 3D architecture adequately. Importantly, the vascular and biliary systems are inter-dependent, and we therefore aimed to develop a robust method to image, digitalize and quantify 3D architecture of the biliary and vascular systems in tandem.

micro computed tomography, biliary system, vascular systém

micro computed tomography, biliary system, vascular systém

HANKEOVÁ, S.; ZIKMUND, T.; KAVKOVÁ, M.; BŘÍNEK, A.; LENDAHL, U.; KAISER, J.; BRYJA, V.; ANDERSSON, E.

2021

01.04.2018

ELSEVIER

AMSTERDAM

1600-0641

JOURNAL OF HEPATOLOGY

68

1

Kingdom of the Netherlands

S414

S415

2