Toxicity assessment of photon-upconversion nanoparticles and their bioimaging by using Laser-Induced Breakdown Spectroscopy in Brassica Oleracea L. plant

Toxicity assessment of photon-upconversion nanoparticles and their bioimaging by using Laser-Induced Breakdown Spectroscopy in Brassica Oleracea L. plant

Abstract

The increasing use of many types of nanoparticles (NPs) in commercial products as well as in many research areas has been found to lead to the NP-accumulation in the environment and within the food chain. Or additionally to an unknown toxic effect at various organism/tissue/cellular levels. The evaluation of NPs toxicity, bioaccumulation, and translocation in diverse organisms is an extremely challenging task [Modlitbová 2020]. The precise localization of NPs through the plant tissues is of a paramount importance in order to reveal the relationship between the exact location of NPs and its toxic effect. In this study, the rare-earth elements-doped photon-upconversion nanoparticles (UCNPs) are studied. They are composed of NaYF4 nanocrystal and doped with Yb3+ and Tm3+ ions; a carboxylated silica shell is added on the surface to increase the chemical stability and reduce the releasing of free ions. Brassica oleracea plant was exposed to the selected contaminant, UCNPs dispersion in the nominal concentration 50 µg UCNPs/mL. After a 72-h exposure, we monitored several macroscopic end-points, and plants were dried, molded, and epoxide glued on the glass slides. The LIBS method served to determine the permeation of Y, Yb, or Tm through the whole plant. The photon-upconversion laser microscaning was used as a complementary technique confirming the presence of Y, Yb, and Tm in the plants in the form of UCNPs .

The increasing use of many types of nanoparticles (NPs) in commercial products as well as in many research areas has been found to lead to the NP-accumulation in the environment and within the food chain. Or additionally to an unknown toxic effect at various organism/tissue/cellular levels. The evaluation of NPs toxicity, bioaccumulation, and translocation in diverse organisms is an extremely challenging task [Modlitbová 2020]. The precise localization of NPs through the plant tissues is of a paramount importance in order to reveal the relationship between the exact location of NPs and its toxic effect. In this study, the rare-earth elements-doped photon-upconversion nanoparticles (UCNPs) are studied. They are composed of NaYF4 nanocrystal and doped with Yb3+ and Tm3+ ions; a carboxylated silica shell is added on the surface to increase the chemical stability and reduce the releasing of free ions. Brassica oleracea plant was exposed to the selected contaminant, UCNPs dispersion in the nominal concentration 50 µg UCNPs/mL. After a 72-h exposure, we monitored several macroscopic end-points, and plants were dried, molded, and epoxide glued on the glass slides. The LIBS method served to determine the permeation of Y, Yb, or Tm through the whole plant. The photon-upconversion laser microscaning was used as a complementary technique confirming the presence of Y, Yb, and Tm in the plants in the form of UCNPs .

Brassica Oleracea; Laser-Induced Breakdown Spectrosopy; Photon-Upconversion nanoparticles

Brassica Oleracea; Laser-Induced Breakdown Spectrosopy; Photon-Upconversion nanoparticles

STŘÍTEŽSKÁ, S.; MODLITBOVÁ, P.; POŘÍZKA, P.; KAISER, J.

2021

02.12.2020

Szeged, Hungary

978-963-306-765-9

International workshop on LIBS

117

120

4

http://www2.sci.u-szeged.hu/libsconf/index_htm_files/libsconf2020_book_of_abstracts_final.pdf