Publication detail

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

JANOUŠEK, O. RONZHINA, M. HEJČ, J. OLEJNÍČKOVÁ, V. STRAČINA, T. FIALOVÁ, K. NOVÁKOVÁ, M. PROVAZNÍK, I. KOLÁŘOVÁ, J.

Original Title

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Type

conference paper

Language

English

Original Abstract

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis.

Keywords

di-4-ANEPPS, HRV, voltage-sensitive dyes, heart rate variability, isolated heart, rabbit heart

Authors

JANOUŠEK, O.; RONZHINA, M.; HEJČ, J.; OLEJNÍČKOVÁ, V.; STRAČINA, T.; FIALOVÁ, K.; NOVÁKOVÁ, M.; PROVAZNÍK, I.; KOLÁŘOVÁ, J.

RIV year

2015

Released

1. 11. 2015

Location

Nice, Francie

ISBN

978-1-4799-4347-0

Book

Computing in Cardiology 2015

Pages from

1049

Pages to

1052

Pages count

4

URL

https://ieeexplore.ieee.org/document/7411094

BibTex

@inproceedings{BUT118271,
  author="Oto {Janoušek} and Marina {Filipenská} and Jakub {Hejč} and Veronika {Olejníčková} and Tibor {Stračina} and Kateřina {Fialová} and Marie {Nováková} and Valentine {Provazník} and Jana {Kolářová}",
  title="The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart",
  booktitle="Computing in Cardiology 2015",
  year="2015",
  pages="1049--1052",
  address="Nice, Francie",
  doi="10.1109/CIC.2015.7411094",
  isbn="978-1-4799-4347-0",
  url="https://ieeexplore.ieee.org/document/7411094"
}