DEVELOPMENT OF CAPILLARY ZONE ELECTROPHORESIS METHOD FOR MONITORING OF SELECTED ORGANIC ACIDS IN FERMENTED FOODS

abstract

English

During processing of food matrix by microorganisms, the great number of various metabolites is formed. Simple organic acids (such as acetic acid, lactic acid etc.) belong among the most important ones. Therefore, monitoring of their production during fermentation process provides valuable information for control of biotechnological processes. Moreover, organic acids have a significant impact on sensory properties, stability of food and are an indicator of microbial contamination. The link between the different fermentation processes is, for example, lactic acid. It is usually formed during fermentation of grape must as well as in the production of fermented dairy products. Lactic acid shows a different perspective on the nature of organic acids. They are either desirable (for the production of milk fermented products), or undesirable - as an indicator of contaminant microflora (lactic fermentation of grape must, undesirable milk souring). Determination of organic acids requires a sensitive analytical method. Capillary zone electrophoresis (CZE) is a suitable method for the determination of these acids, because acids are easily ionizable and CZE is an ecological and economical method, have low exigencies to quantity of samples. Method is fast, accurate and sensitive to low concentrations of analytes. The work is focused to develop a sensitive method for the determination of selected low molecular weight organic acids by capillary zone electrophoresis in wines, wine must and products of lactic fermentation. The aim of work is to find the optimal separation system for the rapid routine determination of selected acids. The acids were separated under slightly alkaline conditions and detected by directly by UV detector (214 nm). The capillary length was 60 cm.

CZE, organic acids, foods, development method

DVOŘÁK, M.; VESPALCOVÁ, M.; RITTICH, B.

17. 8. 2011

Česká společnost chemická

Praha

0009-2770

Chemické listy

105

18

Czech Republic

s1021

s1021

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