Publication detail

Concanavalin A lectin-based affinity chromatography for enrichment and determination of glycoproteins in barley malt

BENKOVSKÁ, D. FLODROVÁ, D. BOBÁĽOVÁ, J.

Original Title

Concanavalin A lectin-based affinity chromatography for enrichment and determination of glycoproteins in barley malt

Type

abstract

Language

English

Original Abstract

In this study, glycoproteins of barley malt were investigated. Glycosylated proteins were first separated from non-glycosylated proteins using a new HPLC monolithic ConA affinity column (Dionex), then separated on SDS-PAGE, digested and identified using MALDI-TOF/TOF mass spectrometer.

Keywords

glycoprotein, glycosylation, concanavalin A, barley, malt, MALDI

Authors

BENKOVSKÁ, D.; FLODROVÁ, D.; BOBÁĽOVÁ, J.

Released

14. 9. 2011

ISBN

0009-2770

Periodical

Chemické listy

Year of study

105

Number

18

State

Czech Republic

Pages from

s1008

Pages to

s1009

Pages count

2