Publication detail

3D printed bone scaffolds coated with bioactive proteins for regenerative medicine

IZSÁK, D. PAVLIŇÁKOVÁ, V. LYSÁKOVÁ, K. MENČÍK, P. KADLECOVÁ, Z. VOJTOVÁ, L.

Original Title

3D printed bone scaffolds coated with bioactive proteins for regenerative medicine

Type

abstract

Language

English

Original Abstract

Large bone defects present a significant challenge due to their slow healing process. However, the use of 3D printing represents a convincing solution compared to traditional hand-processed and cast materials, mainly due to ability to precisely define the shape, size, and morphology of the final product. This innovative technique enables reliable, precise, and cost-effective production of bone substitutes for regenerative medicine. In this project, a bio-ink consisting of calcium phosphate ceramic powder (CaP) modified with thixotropic biodegradable thermosensitive copolymer and biocompatible polysaccharide was prepared to create a non-toxic and fully bioresorbable material for the human body. The prepared bio-ink paste exhibits thixotropic properties, which enable its transform into a printable biomaterial suitable for direct ink writing (DIW) technology. The scaffolds were created using a biomimetic approach. This method involves 3D printing at low temperatures. Bioactive proteins enhancing regeneration or suppressing infection were incorporated into the printed scaffolds to improve the healing process. The proteins were added post-printing using a coating process with a protein solution. Subsequently, the scaffolds were hardened in an environment with high humidity. Protein release was performed at body temperature in a physiological solution (phosphate buffer saline) and monitored using enzyme-linked immunosorbent assay (ELISA). Finally, the structural and morphological characteristics of the printed samples produced at different extrusion coefficients were evaluated using scanning electron microscopy. This analysis provided insights into the variations in structure and morphology induced by the different printing conditions.

Keywords

3D printing, release, protein coating, extrusion coefficient, morphology, scaffolds, regenerative medicine, bone cement, bio-ink

Authors

IZSÁK, D.; PAVLIŇÁKOVÁ, V.; LYSÁKOVÁ, K.; MENČÍK, P.; KADLECOVÁ, Z.; VOJTOVÁ, L.

Released

20. 8. 2023

Publisher

Materials Research Society of Serbia

Location

Belgrade

ISBN

978-86-919111-8-8

Book

THE TWENTY-FOURTH ANNUAL CONFERENCE YUCOMAT 2023 Program and Book of Abstracts Herceg Novi, September 4-8, 2023

Pages from

110

Pages to

110

Pages count

183

URL

https://mrs-serbia.org.rs/index.php/book-of-abstracts-2023

BibTex

@misc{BUT184662,
  author="Dávid {Izsák} and Veronika {Pavliňáková} and Klára {Lysáková} and Přemysl {Menčík} and Zuzana {Kadlecová} and Lucy {Vojtová}",
  title="3D printed bone scaffolds coated with bioactive proteins for regenerative medicine",
  booktitle="THE TWENTY-FOURTH ANNUAL CONFERENCE YUCOMAT 2023
Program and Book of Abstracts

Herceg Novi, September 4-8, 2023",
  year="2023",
  pages="110--110",
  publisher="Materials Research Society of Serbia",
  address="Belgrade",
  isbn="978-86-919111-8-8",
  url="https://mrs-serbia.org.rs/index.php/book-of-abstracts-2023",
  note="abstract"
}