Publication detail

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

SEDLÁČEK, I. HOLOCHOVÁ, P. SOBOTKA, R. BUSSE, H. ŠVEC, P. KRÁLOVÁ, S. ŠEDO, O. PILNÝ, J. STAŇKOVÁ, E. KOUBLOVÁ, V. SEDLÁŘ, K.

Original Title

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Type

journal article in Web of Science

Language

English

Original Abstract

A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900T were Rugamonas rubra CCM 3730T, Rugamonas rivuli FT103WT, and Rugamonas aquatica FT29WT, exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900T represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1 mol%. The major components in fatty acid profiles were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C 16:0, and C12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine.

Keywords

psychrophiles, Rugamonas, taxonomy, Antarctica, violacein, species description

Authors

SEDLÁČEK, I.; HOLOCHOVÁ, P.; SOBOTKA, R.; BUSSE, H.; ŠVEC, P.; KRÁLOVÁ, S.; ŠEDO, O.; PILNÝ, J.; STAŇKOVÁ, E.; KOUBLOVÁ, V.; SEDLÁŘ, K.

Released

11. 8. 2021

Publisher

American Society for Microbiology

ISBN

2165-0497

Periodical

Microbiology spectrum

Year of study

9

Number

1

State

United States of America

Pages from

1

Pages to

14

Pages count

14

URL

https://journals.asm.org/doi/10.1128/Spectrum.00452-21

Full text in the Digital Library

http://hdl.handle.net/11012/201481

BibTex

@article{BUT172213,
  author="Ivo {Sedláček} and Pavla {Holochová} and Roman {Sobotka} and Hans-Jürgen {Busse} and Pavel {Švec} and Stanislava {Králová} and Ondrej {Šedo} and Jan {Pilný} and Eva {Staňková} and Vendula {Koublová} and Karel {Sedlář}",
  title="Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.",
  journal="Microbiology spectrum",
  year="2021",
  volume="9",
  number="1",
  pages="1--14",
  doi="10.1128/Spectrum.00452-21",
  issn="2165-0497",
  url="https://journals.asm.org/doi/10.1128/Spectrum.00452-21"
}