Publication detail

Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

KAVKOVÁ, M. ZIKMUND, T. KALA, A. ŠALPLACHTA, J. LISSETTE PROSKAUER PENA, S. KAISER, J. JEŽEK, K.

Original Title

Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

Type

journal article in Web of Science

Language

English

Original Abstract

Amyloid plaques are small (similar to 50 mu m), highly-dense aggregates of amyloid beta (A beta) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer's disease (AD). Plaques' in vivo detection, spatial distribution and quantitative characterization could be an essential marker in diagnostics and evaluation of AD progress. However, current imaging methods in clinics possess substantial limits in sensitivity towards A beta plaques to play a considerable role in AD screening. Contrast enhanced X-ray micro computed tomography (micro CT) is an emerging highly sensitive imaging technique capable of high resolution visualization of rodent brain. In this study we show the absorption based contrast enhanced X-ray micro CT imaging is viable method for detection and 3D analysis of A beta plaques in transgenic rodent models of Alzheimer's disease. Using iodine contrasted brain tissue isolated from the Tg-F344-AD rat model we show the micro CT imaging is capable of precise imaging of A beta plaques, making possible to further analyze various aspects of their 3D spatial distribution and other properties.

Keywords

micro CT, Alzheimers disease, imaging

Authors

KAVKOVÁ, M.; ZIKMUND, T.; KALA, A.; ŠALPLACHTA, J.; LISSETTE PROSKAUER PENA, S.; KAISER, J.; JEŽEK, K.

Released

16. 3. 2021

Publisher

Springer Nature

Location

BERLIN

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

11

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

10

Pages count

10

URL

https://www.nature.com/articles/s41598-021-84579-x

Full text in the Digital Library

http://hdl.handle.net/11012/202272

BibTex

@article{BUT172018,
  author="Michaela {Kavková} and Tomáš {Zikmund} and Annu {Kala} and Jakub {Šalplachta} and Stephanie {Lissette Proskauer Pena} and Jozef {Kaiser} and Karel {Ježek}",
  title="Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system",
  journal="Scientific Reports",
  year="2021",
  volume="11",
  number="1",
  pages="1--10",
  doi="10.1038/s41598-021-84579-x",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-021-84579-x"
}