Publication detail

Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation

HASHEMI, A. EZATI, M. MOHAMMADNEJAD, J. HOUSHMAND, B. FAGHIHI, S.

Original Title

Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Ineffective integration has been recognized as one of the major causes of early orthopedic failure of titanium-based implants. One strategy to address this problem is to develop modified titanium surfaces that promote osteoblast differentiation. This study explored titanium surfaces modified with TiO2 nanotubes (TiO2 NTs) capable of localized drug delivery into bone and enhanced osteoblast cell differentiation. Materials and Methods: Briefly, TiO2 NTs were subjected to anodic oxidation and loaded with Metformin, a widely used diabetes drug. To create surfaces with sustainable drug-eluting characteristics, TiO2 NTs were spin coated with a thin layer of chitosan. The surfaces were characterized via scanning electron microscopy, atomic force microscopy, and contact angle measurements. The surfaces were then exposed to mesenchymal bone marrow stem cells (MSCs) to evaluate cell adhesion, growth, differentiation, and morphology on the modified surfaces. Results: A noticeable increase in drug release time (3 days vs 20 days) and a decrease in burst release characteristics (85% to 7%) was observed in coated samples as compared to uncoated samples, respectively. Chitosan-coated TiO2 NTs exhibited a considerable enhancement in cell adhesion, proliferation, and genetic expression of type I collagen, and alkaline phosphatase activity as compared to uncoated TiO2 NTs. Conclusion: TiO2 NT surfaces with a chitosan coating are capable of delivering Metformin to a bone site over a sustained period of time with the potential to enhance MSCs cell attachment, proliferation, and differentiation.

Keywords

titania nanotubes; titanium; osteogenic differentiation; anodization; mesenchymal bone marrow stem cells; MSCs

Authors

HASHEMI, A.; EZATI, M.; MOHAMMADNEJAD, J.; HOUSHMAND, B.; FAGHIHI, S.

Released

22. 6. 2020

Publisher

DOVE MEDICAL PRESS LTD

Location

ALBANY

ISBN

1178-2013

Periodical

International Journal of Nanomedicine

Year of study

15

Number

1

State

New Zealand

Pages from

4471

Pages to

4481

Pages count

11

URL

https://www.dovepress.com/chitosan-coating-of-tio2-nanotube-arrays-for-improved-metformin-releas-peer-reviewed-fulltext-article-IJN

BibTex

@article{BUT171654,
  author="Amir {Hashemi} and Masoumeh {Ezati} and Javad {Mohammadnejad} and Behzad {Houshmand} and Shahab {Faghihi}",
  title="Chitosan Coating of TiO2 Nanotube Arrays for Improved Metformin Release and Osteoblast Differentiation",
  journal="International Journal of Nanomedicine",
  year="2020",
  volume="15",
  number="1",
  pages="4471--4481",
  doi="10.2147/IJN.S248927",
  issn="1178-2013",
  url="https://www.dovepress.com/chitosan-coating-of-tio2-nanotube-arrays-for-improved-metformin-releas-peer-reviewed-fulltext-article-IJN"
}