Publication result detail

RAPID FABRICATION OF MICROEXTRACTION DEVICES FOR DIRECT DETERMINATION OF BASIC DRUGS IN DRIED BLOOD SPOTS

RYŠAVÁ, L.; PŘIKRYL, J.; DVOŘÁK, M.; MALÁ, Z.; KUBÁŇ, P.

Original Title

RAPID FABRICATION OF MICROEXTRACTION DEVICES FOR DIRECT DETERMINATION OF BASIC DRUGS IN DRIED BLOOD SPOTS

English Title

RAPID FABRICATION OF MICROEXTRACTION DEVICES FOR DIRECT DETERMINATION OF BASIC DRUGS IN DRIED BLOOD SPOTS

Type

Paper in proceedings (conference paper)

Original Abstract

A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 µg/mL.

English abstract

A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 µg/mL.

Keywords

capillary electrophoresis, 3D print cutter, blood spots

Key words in English

capillary electrophoresis, 3D print cutter, blood spots

Authors

RYŠAVÁ, L.; PŘIKRYL, J.; DVOŘÁK, M.; MALÁ, Z.; KUBÁŇ, P.

RIV year

2019

Released

08.10.2018

ISBN

978-80-904959-5-1

Book

CECE 2018: 15th International Interdisciplinary Meeting on Bioanalysis

Pages from

257

Pages to

260

Pages count

4

URL

http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

BibTex

@inproceedings{BUT152127,
  author="Lenka {Ryšavá} and Miloš {Dvořák}",
  title="RAPID FABRICATION OF MICROEXTRACTION DEVICES FOR DIRECT DETERMINATION OF BASIC DRUGS IN DRIED BLOOD SPOTS",
  booktitle="CECE 2018: 15th International Interdisciplinary Meeting on Bioanalysis",
  year="2018",
  pages="257--260",
  isbn="978-80-904959-5-1",
  url="http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf"
}