Publication result detail

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

MANAKHOV, A.; MAKHNEVA, E.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; KALINA, L.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

Original Title

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

English Title

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

Type

WoS Article

Original Abstract

The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiol-based self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications.

English abstract

The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiol-based self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications.

Keywords

Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM

Key words in English

Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM

Authors

MANAKHOV, A.; MAKHNEVA, E.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; KALINA, L.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

RIV year

2017

Released

01.01.2016

ISBN

0169-4332

Periodical

APPLIED SURFACE SCIENCE

Volume

360

Number

Part A

State

Kingdom of the Netherlands

Pages from

28

Pages to

36

Pages count

9

BibTex

@article{BUT119199,
  author="Anton {Manakhov} and Ekaterina {Makhneva} and Petr {Skládal} and David {Nečas} and Jan {Čechal} and Lukáš {Kalina} and Marek {Eliáš} and Lenka {Zajíčková}",
  title="The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry",
  journal="APPLIED SURFACE SCIENCE",
  year="2016",
  volume="360",
  number="Part A",
  pages="28--36",
  doi="10.1016/j.apsusc.2015.10.178",
  issn="0169-4332"
}