Detail publikace

Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects

JANČÁŘ, J. VOJTOVÁ, L. NEČAS, A. SRNEC, R. URBANOVÁ, L. CRHA, M.

Originální název

Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects

Anglický název

Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects

Jazyk

en

Originální abstrakt

Synthesis and characterization of biodegradable hydrogels based on collagen modified by addition of synthetic biodegradable copolymer intended for preparation of porous scaffolds for mesenchymal stem cells used for possible implantation to animals with articular surface defects was investigated. The synthetic biodegradable tri-block copolymer used was the block copolymer of polyethylene glycol (PEG), polylactic acid (PLA), polyglycolic acid (PGA) (PEG-PLGA) endcapped with itaconic acid (ITA). The water-soluble carbodiimide and N-hydroxysuccimide system (EDC-NHS) was chosen as the cross-linking agent used to control the rate of hydrogel resorption. Dependence of the physical properties of the prepared hydrogels on the concentration of the EDC-NHS cross-linker, reaction time and concentration of PEG-PLGA-ITA copolymer was examined. Swelling behaviour, thermal stability, surface morphology and degradation rate were also characterized. Based on the obtained results, it can be concluded that increase in concentration of the cross-linking agent, as well as prolonged cross-linking time and increased amount of synthetic copolymer lead to enhanced thermal stability of the gels together with a reduced swelling ratio and degradation rate in saline. The resorption rate of these gels used in preparation of cartilage scaffolds can be controlled over a wide time interval by varying the collagen/(PEG-PLGA-ITA) blend composition or the conditions of the cross-linking reaction.

Anglický abstrakt

Synthesis and characterization of biodegradable hydrogels based on collagen modified by addition of synthetic biodegradable copolymer intended for preparation of porous scaffolds for mesenchymal stem cells used for possible implantation to animals with articular surface defects was investigated. The synthetic biodegradable tri-block copolymer used was the block copolymer of polyethylene glycol (PEG), polylactic acid (PLA), polyglycolic acid (PGA) (PEG-PLGA) endcapped with itaconic acid (ITA). The water-soluble carbodiimide and N-hydroxysuccimide system (EDC-NHS) was chosen as the cross-linking agent used to control the rate of hydrogel resorption. Dependence of the physical properties of the prepared hydrogels on the concentration of the EDC-NHS cross-linker, reaction time and concentration of PEG-PLGA-ITA copolymer was examined. Swelling behaviour, thermal stability, surface morphology and degradation rate were also characterized. Based on the obtained results, it can be concluded that increase in concentration of the cross-linking agent, as well as prolonged cross-linking time and increased amount of synthetic copolymer lead to enhanced thermal stability of the gels together with a reduced swelling ratio and degradation rate in saline. The resorption rate of these gels used in preparation of cartilage scaffolds can be controlled over a wide time interval by varying the collagen/(PEG-PLGA-ITA) blend composition or the conditions of the cross-linking reaction.

Plný text v Digitální knihovně

Dokumenty

BibTex


@article{BUT47810,
  author="Josef {Jančář} and Lucy {Vojtová} and Alois {Nečas} and Robert {Srnec} and Lucie {Urbanová} and Michal {Crha}",
  title="Stability of collagen scaffold implants for animals with iatrogenic articular cartilage defoects",
  annote="Synthesis and characterization of biodegradable hydrogels based on collagen modified by addition of synthetic biodegradable copolymer intended for preparation of porous scaffolds for mesenchymal stem cells used for possible implantation to animals with articular surface defects was investigated. The synthetic biodegradable tri-block copolymer used was the block copolymer of polyethylene glycol (PEG), polylactic acid (PLA), polyglycolic acid (PGA) (PEG-PLGA) endcapped with itaconic acid (ITA). The water-soluble carbodiimide and N-hydroxysuccimide system (EDC-NHS) was chosen as the cross-linking agent used to control the rate of hydrogel resorption. Dependence of the physical properties of the prepared hydrogels on the concentration of the EDC-NHS cross-linker, reaction time and concentration of PEG-PLGA-ITA copolymer was examined. Swelling behaviour, thermal stability, surface morphology and degradation rate were also characterized. Based on the obtained results, it can be concluded that increase in concentration of the cross-linking agent, as well as prolonged cross-linking time and increased amount of synthetic copolymer lead to enhanced thermal stability of the gels together with a reduced swelling ratio and degradation rate in saline. The resorption rate of these gels used in preparation of cartilage scaffolds can be controlled over a wide time interval by varying the collagen/(PEG-PLGA-ITA) blend composition or the conditions of the cross-linking reaction.",
  address="University of Veterinary and Pharmaceutical Sciences in Brno",
  chapter="47810",
  doi="10.2754/avb200978040643",
  institution="University of Veterinary and Pharmaceutical Sciences in Brno",
  journal="ACTA VETERINARIA BRNO",
  number="4",
  volume="78",
  year="2009",
  month="december",
  pages="643--648",
  publisher="University of Veterinary and Pharmaceutical Sciences in Brno",
  type="journal article - other"
}