Detail publikace

Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water

PÓL, J. VARAĎOVÁ OSTRÁ, E. KARÁSEK, P. ROTH, M. BENEŠOVÁ, K. KOTLAŘÍKOVÁ, P. ČÁSLAVSKÝ, J.

Originální název

Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Pressurised fluid extraction using water or methanol was employed for the extraction of stevioside from Stevia rebaudiana Bertoni. The extraction method was optimised in terms of temperature and duration of the static or the dynamic step. Extracts were analysed by liquid chromatography followed by UV and mass-spectrometric (MS) detections. Thermal degradation of stevioside was the same in both solvents within the range 70 to 160 deg. C. Methanol showed better extraction ability for isolation of stevioside from Stevia rebaudiana leaves than water within the range 110 to 160 deg. C. However, water represents the green alternative to methanol. The limit of detection of stevioside in the extract analysed was 30 ng for UV detection and 2 ng for MS detection.

Klíčová slova

Pressurised fluid extraction; Liquid chromatography / Mass spectrometry; Stevia rebaudiana; Stevioside

Autoři

PÓL, J.; VARAĎOVÁ OSTRÁ, E.; KARÁSEK, P.; ROTH, M.; BENEŠOVÁ, K.; KOTLAŘÍKOVÁ, P.; ČÁSLAVSKÝ, J.

Rok RIV

2007

Vydáno

15. 8. 2007

Nakladatel

Springer

Místo

Berlin - Heidelberg

ISSN

1618-2642

Periodikum

Analytical and Bioanalytical Chemistry

Ročník

388

Číslo

8

Stát

Spolková republika Německo

Strany od

1847

Strany do

1857

Strany počet

11

BibTex

@article{BUT43961,
  author="Jaroslav {Pól} and Elena {Ostrá} and Pavel {Karásek} and Michal {Roth} and Karolína {Benešová} and Pavla {Kožušníková} and Josef {Čáslavský}",
  title="Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water",
  journal="Analytical and Bioanalytical Chemistry",
  year="2007",
  volume="388",
  number="8",
  pages="1847--1857",
  issn="1618-2642"
}