Detail publikace

Increasing the Image Contrast via Fast Fluorescence Photobleaching

KOLÁŘ, R. VIČAR, T. CHMELÍKOVÁ, L. PROVAZNÍK, I.

Originální název

Increasing the Image Contrast via Fast Fluorescence Photobleaching

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

This paper focuses on the analysis of image sequences acquired during fast photobleaching using a standard wide-field microscope. We show that the photobleaching rate estimated for each pixel is not constant for the whole field of view, but it provides a new spatially variant parametric image related to the cell structure and diffusion of fluorophores. We also provide an alternative way to estimate a pixel-wise photobleaching rate with significantly less computation time than exponential model fitting.

Klíčová slova

image sequences;photobleaching;microscopy

Autoři

KOLÁŘ, R.; VIČAR, T.; CHMELÍKOVÁ, L.; PROVAZNÍK, I.

Vydáno

18. 11. 2021

Nakladatel

Elsevier

ISBN

978-1-7281-1179-7

Kniha

2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)

Strany od

2741

Strany do

2744

Strany počet

4

URL

https://ieeexplore.ieee.org/document/9630859

BibTex

@inproceedings{BUT173256,
  author="Radim {Kolář} and Tomáš {Vičar} and Larisa {Chmelíková} and Valentine {Provazník}",
  title="Increasing the Image Contrast via Fast Fluorescence Photobleaching",
  booktitle="2021 43nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)",
  year="2021",
  pages="2741--2744",
  publisher="Elsevier",
  doi="10.1109/EMBC46164.2021.9630859",
  isbn="978-1-7281-1179-7",
  url="https://ieeexplore.ieee.org/document/9630859"
}