Detail publikace

Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

SEDLÁŘ, K. NYKRÝNOVÁ, M. BEZDÍČEK, M. BRANSKÁ, B. LENGEROVÁ, M. PATÁKOVÁ, P. ŠKUTKOVÁ, H.

Originální název

Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.

Klíčová slova

butanol; ABE; IBE; core genome; accessory genome; pan genome

Autoři

SEDLÁŘ, K.; NYKRÝNOVÁ, M.; BEZDÍČEK, M.; BRANSKÁ, B.; LENGEROVÁ, M.; PATÁKOVÁ, P.; ŠKUTKOVÁ, H.

Vydáno

10. 7. 2021

Nakladatel

MDPI

ISSN

2227-9717

Periodikum

Processes

Ročník

9

Číslo

7

Stát

Švýcarská konfederace

Strany od

1

Strany do

16

Strany počet

16

URL

https://www.mdpi.com/2227-9717/9/7/1196

Plný text v Digitální knihovně

http://hdl.handle.net/11012/200480

BibTex

@article{BUT171987,
  author="Karel {Sedlář} and Markéta {Nykrýnová} and Matěj {Bezdíček} and Barbora {Branská} and Martina {Lengerová} and Petra {Patáková} and Helena {Vítková}",
  title="Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T",
  journal="Processes",
  year="2021",
  volume="9",
  number="7",
  pages="1--16",
  doi="10.3390/pr9071196",
  issn="2227-9717",
  url="https://www.mdpi.com/2227-9717/9/7/1196"
}

Dokumenty

processes-09-01196.pdf