Detail publikace

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)

SEDLAČÍK, T. ACAR, O. STUDENOVSKÁ, H. KOTELNIKOV, I. KUČKA, J. KONEČNÁ, Z. ZIKMUND, T. KAISER, J. KOSE, G. RYPÁČEK, F.

Originální název

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(a-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxyethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cellsupporting moieties (RGDS peptides) by the azide–alkyne ‘‘click’’-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.

Klíčová slova

cryogel, polyamino acid

Autoři

SEDLAČÍK, T.; ACAR, O.; STUDENOVSKÁ, H.; KOTELNIKOV, I.; KUČKA, J.; KONEČNÁ, Z.; ZIKMUND, T.; KAISER, J.; KOSE, G.; RYPÁČEK, F.

Vydáno

30. 10. 2017

ISSN

1744-683X

Periodikum

Soft Matter

Číslo

14

Stát

Spojené království Velké Británie a Severního Irska

Strany od

228

Strany do

238

Strany počet

11

BibTex

@article{BUT146251,
  author="Tomáš {Sedlačík} and O.K. {Acar} and Hana {Studenovská} and Ilyia {Kotelnikov} and Jan {Kučka} and Zuzana {Konečná} and Tomáš {Zikmund} and Jozef {Kaiser} and G.T. {Kose} and František {Rypáček}",
  title="Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)",
  journal="Soft Matter",
  year="2017",
  number="14",
  pages="228--238",
  doi="10.1039/c7sm02074k",
  issn="1744-683X"
}