Detail publikace

Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

SLANINOVÁ, E. SEDLÁČEK, P. MRAVEC, F. MÜLLEROVÁ, L. SAMEK, O. KOLLER, M. HESKO, O. KUČERA, D. MÁROVÁ, I. OBRUČA, S.

Originální název

Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments.

Klíčová slova

Polyhydroxyalkanoates Cupriavidus necator UV radiation Turbidity Integrating sphere Nephelometry

Autoři

SLANINOVÁ, E.; SEDLÁČEK, P.; MRAVEC, F.; MÜLLEROVÁ, L.; SAMEK, O.; KOLLER, M.; HESKO, O.; KUČERA, D.; MÁROVÁ, I.; OBRUČA, S.

Vydáno

19. 1. 2018

Nakladatel

Springer

Místo

Springer Berlin Heidelberg

ISSN

1432-0614

Periodikum

Applied Microbiology and Biotechnology

Ročník

102

Číslo

4

Stát

Spolková republika Německo

Strany od

1923

Strany do

1931

Strany počet

9

URL

https://link.springer.com/article/10.1007/s00253-018-8760-8

BibTex

@article{BUT145891,
  author="SLANINOVÁ, E. and SEDLÁČEK, P. and MRAVEC, F. and MÜLLEROVÁ, L. and SAMEK, O. and KOLLER, M. and HESKO, O. and KUČERA, D. and MÁROVÁ, I. and OBRUČA, S.",
  title="Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation",
  journal="Applied Microbiology and Biotechnology",
  year="2018",
  volume="102",
  number="4",
  pages="1923--1931",
  doi="10.1007/s00253-018-8760-8",
  issn="1432-0614",
  url="https://link.springer.com/article/10.1007/s00253-018-8760-8"
}

Dokumenty

cena za nejlepší poster.pdf
článek light scatteing on PHA granules protects bacterial cells against the harmful effects of UV radiation.pdf