Detail publikace

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

MANAKHOV, A. MAKHNEVA, E. SKLÁDAL, P. NEČAS, D. ČECHAL, J. KALINA, L. ELIÁŠ, M. ZAJÍČKOVÁ, L.

Originální název

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiol-based self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications.

Klíčová slova

Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM

Autoři

MANAKHOV, A.; MAKHNEVA, E.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; KALINA, L.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

Vydáno

1. 1. 2016

ISSN

0169-4332

Periodikum

Applied Surface Science

Ročník

360

Číslo

Part A

Stát

Nizozemsko

Strany od

28

Strany do

36

Strany počet

9

BibTex

@article{BUT119199,
  author="Anton {Manakhov} and Ekaterina {Makhneva} and Petr {Skládal} and David {Nečas} and Jan {Čechal} and Lukáš {Kalina} and Marek {Eliáš} and Lenka {Zajíčková}",
  title="The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry",
  journal="Applied Surface Science",
  year="2016",
  volume="360",
  number="Part A",
  pages="28--36",
  doi="10.1016/j.apsusc.2015.10.178",
  issn="0169-4332"
}