Advanced Fluorescence Spectroscopy Techniques as a Tool for Studying the Interaction of New Types of Vesicular Systems with Hydrophobised Hyaluronan Derivatives

Advanced Fluorescence Spectroscopy Techniques as a Tool for Studying the Interaction of New Types of Vesicular Systems with Hydrophobised Hyaluronan Derivatives

Abstrakt

This contribution investigates the interaction between native or hydrophobically modified hyaluronan and vesicular systems using advanced fluorescence techniques to monitor subtle changes in membrane organisation and physicochemical properties. The primary focus is to compare the effect of native hyaluronan versus hydrophobically modified oleyl-hyaluronan on the structural integrity and microdynamics of 4 vesicular systems: standard DPPC liposomes and DOPE liposomes and two modifications of ion-pair amphiphiles (IPA), with and without cholesterol. These vesicles may serve as an alternative to conventional liposomes[1]. The research focuses on the potential of fluorescent probes to report changes in system properties arising from complex interactions between the host membranes of vesicular systems and polymers. While native hyaluronan acts primarily as a hydrophilic coating, the oleyl-derivative introduces hydrophobic anchors that can penetrate the vesicular bilayers[2]. The fluidity of the membranes and the effect of temperature on it were determined using the fluorescence anisotropy technique of the 1,6-diphenyl-1,3,5-hexatriene (DPH) probe, and the membrane hydration was determined using the generalised polarisation (GP) of the Laurdan probe[3]. To gain a deeper perspective in the time domain, time-resolved emission spectroscopy (TRES) was used. This technique allows the calculation of solvent relaxation parameters and the analysis of time-resolved area-normalised emission spectra (TRANES), which provides information on the homogeneity or heterogeneity of the microenvironment and the presence of water molecules in the membrane[4]. For a comprehensive description of the prepared systems and their complexes with polymers, the sizes and polydispersity index were also determined by the dynamic light scattering (DLS) method and their colloidal stability, specifically the zeta potential values, by the electrophoretic light scattering (ELS) method. REFERENCES [1] Richard C., Cassel S., Blanzat M.: RSC Adv. 11, 442 (2021). [2] Zhu J., Xu L., Wang W., Xiao M., Li J., Wang L., Jiang X.: ACS Omega 9, 33833 (2024). [3] Havlíková M., Szabová J., Mravcová L., Venerová T., Chang C. H., Pekař M.: Langmuir 37, 2436 (2021). [4] Rama S., Krishna M. B., Periasamy N.: J. Phys. Chem. A 105, 1767 (2001).

This contribution investigates the interaction between native or hydrophobically modified hyaluronan and vesicular systems using advanced fluorescence techniques to monitor subtle changes in membrane organisation and physicochemical properties. The primary focus is to compare the effect of native hyaluronan versus hydrophobically modified oleyl-hyaluronan on the structural integrity and microdynamics of 4 vesicular systems: standard DPPC liposomes and DOPE liposomes and two modifications of ion-pair amphiphiles (IPA), with and without cholesterol. These vesicles may serve as an alternative to conventional liposomes[1]. The research focuses on the potential of fluorescent probes to report changes in system properties arising from complex interactions between the host membranes of vesicular systems and polymers. While native hyaluronan acts primarily as a hydrophilic coating, the oleyl-derivative introduces hydrophobic anchors that can penetrate the vesicular bilayers[2]. The fluidity of the membranes and the effect of temperature on it were determined using the fluorescence anisotropy technique of the 1,6-diphenyl-1,3,5-hexatriene (DPH) probe, and the membrane hydration was determined using the generalised polarisation (GP) of the Laurdan probe[3]. To gain a deeper perspective in the time domain, time-resolved emission spectroscopy (TRES) was used. This technique allows the calculation of solvent relaxation parameters and the analysis of time-resolved area-normalised emission spectra (TRANES), which provides information on the homogeneity or heterogeneity of the microenvironment and the presence of water molecules in the membrane[4]. For a comprehensive description of the prepared systems and their complexes with polymers, the sizes and polydispersity index were also determined by the dynamic light scattering (DLS) method and their colloidal stability, specifically the zeta potential values, by the electrophoretic light scattering (ELS) method. REFERENCES [1] Richard C., Cassel S., Blanzat M.: RSC Adv. 11, 442 (2021). [2] Zhu J., Xu L., Wang W., Xiao M., Li J., Wang L., Jiang X.: ACS Omega 9, 33833 (2024). [3] Havlíková M., Szabová J., Mravcová L., Venerová T., Chang C. H., Pekař M.: Langmuir 37, 2436 (2021). [4] Rama S., Krishna M. B., Periasamy N.: J. Phys. Chem. A 105, 1767 (2001).

MÁLKOVÁ, I.; MRAVEC, F.

12.05.2026

Czech Chemical Society

Praha

Czech Chemical Society Symposium Series

24

2

Česká republika

163

56

http://www.ccsss.cz/index.php/ccsss/issue/view/55