Detail publikačního výsledku

Exploring the Benefits of SaOS-2 and HUVEC Co-Culture on Collagen Scaffolds for Bone Healing

ŠEBOVÁ, E.; KLUSÁČEK RAMPICHOVÁ, M.; PAVLIŇÁKOVÁ, V.; VOJTOVÁ, L.; FILOVÁ, E.

Originální název

Exploring the Benefits of SaOS-2 and HUVEC Co-Culture on Collagen Scaffolds for Bone Healing

Anglický název

Exploring the Benefits of SaOS-2 and HUVEC Co-Culture on Collagen Scaffolds for Bone Healing

Druh

Abstrakt

Originální abstrakt

This study investigates the efficacy of Collagen foams and collagen/HA composite foams in promoting osteogenic differentiation of SaOS-2 cells and angiogenic potential in HUVECs, hypothesizing that the incorporation of HA will enhance these cellular responses. Cell viability was assessed using the MTS assay, while alkaline phosphatase (ALP) activity and gene expression analysis of osteogenic markers (COL1, BGLAP) were employed to evaluate osteogenic differentiation. Cellular proliferation was determined by quantifying DNA content using the Quant-iT PicoGreen assay. Gene expression analysis was also performed to assess the expression of endothelial cell markers (PECAM1 and KDR). Confocal microscopy was utilized to visualize cellular morphology and investigate cell-biomaterial interactions.

Anglický abstrakt

This study investigates the efficacy of Collagen foams and collagen/HA composite foams in promoting osteogenic differentiation of SaOS-2 cells and angiogenic potential in HUVECs, hypothesizing that the incorporation of HA will enhance these cellular responses. Cell viability was assessed using the MTS assay, while alkaline phosphatase (ALP) activity and gene expression analysis of osteogenic markers (COL1, BGLAP) were employed to evaluate osteogenic differentiation. Cellular proliferation was determined by quantifying DNA content using the Quant-iT PicoGreen assay. Gene expression analysis was also performed to assess the expression of endothelial cell markers (PECAM1 and KDR). Confocal microscopy was utilized to visualize cellular morphology and investigate cell-biomaterial interactions.

Klíčová slova

Collagen, hydroxyapatite, MTS test, gene expresion

Klíčová slova v angličtině

Collagen, hydroxyapatite, MTS test, gene expresion

Autoři

ŠEBOVÁ, E.; KLUSÁČEK RAMPICHOVÁ, M.; PAVLIŇÁKOVÁ, V.; VOJTOVÁ, L.; FILOVÁ, E.

Vydáno

06.05.2025

Místo

Vanajanlinna, Hämeenlinna, Finland

Strany od

4

Strany do

4

Strany počet

12

URL

https://events.tuni.fi/scsb2025/programme/

BibTex

@misc{BUT200630,
  author="Eva {Šebová} and  {} and Michala {Klusáček Rampichová} and  {} and Veronika {Pavliňáková} and  {} and Lucy {Vojtová} and  {} and Eva {Filová} and  {} and  {} and  {} and  {} and  {} and  {} and  {} and  {} and  {} and  {} and  {}",
  title="Exploring the Benefits of SaOS-2 and HUVEC Co-Culture on Collagen Scaffolds for Bone Healing",
  year="2025",
  pages="12",
  address="Vanajanlinna, Hämeenlinna, Finland",
  url="https://events.tuni.fi/scsb2025/programme/",
  note="Abstract"
}