In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel

In vitro culture platform based on electroconductive BSA/PEDOT:PSS hydrogel

Abstrakt

The development of conductive hydrogel-based platforms combined with optics for studying cardiomyocytes and their interactions in vitro is of a great attention. Complementing the use of these platforms with computer modeling represents an innovative and unique approach for studying cardiac cellular electrophysiology and pathophysiology. The conductive hydrogel is prepared from natural bovine serum albumin (BSA) and conducting PEDOT:PSS polymer. The work involves preparation of hydrogels with desired mechanico-electrical properties, physico-chemical characterization of hydrogel and biocompatibility to cardiac cells. The preliminary results shows that BSA/PEDOT:PSS creates the optically transparent hydrogel with Young modulus and ionic conductivity about 20 kPa and 10-4 S·m, respectively. The hydrogel als shows good adhesion and non-toxicity to cardiac HL-1 cells. The proposed novel in vitro culture platforms can be used to study phenomena previously undiscoverable with electrically insulating cell culture substrates.

The development of conductive hydrogel-based platforms combined with optics for studying cardiomyocytes and their interactions in vitro is of a great attention. Complementing the use of these platforms with computer modeling represents an innovative and unique approach for studying cardiac cellular electrophysiology and pathophysiology. The conductive hydrogel is prepared from natural bovine serum albumin (BSA) and conducting PEDOT:PSS polymer. The work involves preparation of hydrogels with desired mechanico-electrical properties, physico-chemical characterization of hydrogel and biocompatibility to cardiac cells. The preliminary results shows that BSA/PEDOT:PSS creates the optically transparent hydrogel with Young modulus and ionic conductivity about 20 kPa and 10-4 S·m, respectively. The hydrogel als shows good adhesion and non-toxicity to cardiac HL-1 cells. The proposed novel in vitro culture platforms can be used to study phenomena previously undiscoverable with electrically insulating cell culture substrates.

condictive hydrogel, in vitro testing

condictive hydrogel, in vitro testing

FOHLEROVÁ, Z.; POLÁKOVÁ, V.

02.07.2024

Greece, Thessaloniki

21st International Conference on Nanosciences & Nanotechnologies, Book of Abstract, Greece, Thessaloniki, 2-5 July 2024

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https://www.nanotexnology.com/index.php/abstract-books-2024/send/5-abstract-books-2024/16-nn24-abstract-book