Detail publikačního výsledku

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

Li, Zhonghe; Gao, Xingai; Li, Ming; Yan, Qiuliang; Zhang, Nan; Yu, Boyang; Zhang, Bimi; Zhang, Shuying; Helal, Mohamed H.; Ali, Ola A. Abu; Nassan, Mohamed A.; Qyyum, Muhammad Abdul; Asif, Saira; Bokhari, Awais

Originální název

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

Anglický název

Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

Druh

Článek WoS

Originální abstrakt

Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.

Anglický abstrakt

Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.

Klíčová slova

Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water; BACTERIAL BIOSENSORS; DRINKING-WATER; ESTROGENS; BIOAVAILABILITY; CONSTRUCTION; EXTRACTION; SENSORS; RIVER; ASSAY

Klíčová slova v angličtině

Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water; BACTERIAL BIOSENSORS; DRINKING-WATER; ESTROGENS; BIOAVAILABILITY; CONSTRUCTION; EXTRACTION; SENSORS; RIVER; ASSAY

Autoři

Li, Zhonghe; Gao, Xingai; Li, Ming; Yan, Qiuliang; Zhang, Nan; Yu, Boyang; Zhang, Bimi; Zhang, Shuying; Helal, Mohamed H.; Ali, Ola A. Abu; Nassan, Mohamed A.; Qyyum, Muhammad Abdul; Asif, Saira; Bokhari, Awais

Rok RIV

2023

Vydáno

01.12.2022

Nakladatel

ACADEMIC PRESS INC ELSEVIER SCIENCE

Místo

San Diego

ISSN

0013-9351

Periodikum

Environmental Research

Svazek

2

Číslo

215

Stát

Spojené státy americké

Strany počet

7

URL

https://www.sciencedirect.com/science/article/pii/S0013935122016309?via%3Dihub

BibTex

@article{BUT182642,
  author="Li, Zhonghe and Gao, Xingai and Li, Ming and Yan, Qiuliang and Zhang, Nan and Yu, Boyang and Zhang, Bimi and Zhang, Shuying and Helal, Mohamed H. and Ali, Ola A. Abu and Nassan, Mohamed A. and Qyyum, Muhammad Abdul and Asif, Saira and Bokhari, Awais",
  title="Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application",
  journal="Environmental Research",
  year="2022",
  volume="2",
  number="215",
  pages="7",
  doi="10.1016/j.envres.2022.114303",
  issn="0013-9351",
  url="https://www.sciencedirect.com/science/article/pii/S0013935122016309?via%3Dihub"
}