Detail publikačního výsledku

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

SVOBODA, O.; FOHLEROVÁ, Z.; BAIAZITOVA, L.; ČMIEL, V.; HUBÁLEK, J.; PROVAZNÍK, I.; PEKAŘÍK, V.

Originální název

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Anglický název

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Druh

Stať ve sborníku mimo WoS a Scopus

Originální abstrakt

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

Anglický abstrakt

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

Klíčová slova

transfection, MATRA-A reagent, plasmid delivery

Klíčová slova v angličtině

transfection, MATRA-A reagent, plasmid delivery

Autoři

SVOBODA, O.; FOHLEROVÁ, Z.; BAIAZITOVA, L.; ČMIEL, V.; HUBÁLEK, J.; PROVAZNÍK, I.; PEKAŘÍK, V.

Rok RIV

2017

Vydáno

06.05.2016

Nakladatel

Masarykova Univerzita - MUNI PRESS

Místo

Brno, Czech Republic

ISBN

978-80-210-8267-0

Kniha

XVI. Workshop of Physical Chemists and Electrochemists

Edice

1

Strany od

1

Strany do

3

Strany počet

3

BibTex

@inproceedings{BUT128268,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Chmelíková} and Vratislav {Čmiel} and Jaromír {Hubálek} and Valentýna {Provazník} and Vladimír {Pekařík}",
  title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery",
  booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
  year="2016",
  series="1",
  pages="1--3",
  publisher="Masarykova Univerzita - MUNI PRESS",
  address="Brno, Czech Republic",
  isbn="978-80-210-8267-0"
}