Detail publikace

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

ŠTRBKOVÁ, L. MANAKHOV, A. ZAJÍČKOVÁ, L. STOICA, A. VESELÝ, P. CHMELÍK, R.

Originální název

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation.

Klíčová slova

Plasma polymer; cyclopropylamine; cell adhesion; holographic microscopy; quantitative phase imaging

Autoři

ŠTRBKOVÁ, L.; MANAKHOV, A.; ZAJÍČKOVÁ, L.; STOICA, A.; VESELÝ, P.; CHMELÍK, R.

Vydáno

15. 6. 2016

Nakladatel

Elsevier B.V.

ISSN

0257-8972

Periodikum

Surface and Coatings Technology

Ročník

295

Číslo

-

Stát

Švýcarská konfederace

Strany od

70

Strany do

77

Strany počet

8

URL

http://www.sciencedirect.com/science/article/pii/S0257897215303728

Plný text v Digitální knihovně

http://hdl.handle.net/11012/84148

BibTex

@article{BUT120405,
  author="Lenka {Štrbková} and Anton {Manakhov} and Lenka {Zajíčková} and Adrian {Stoica} and Pavel {Veselý} and Radim {Chmelík}",
  title="The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy",
  journal="Surface and Coatings Technology",
  year="2016",
  volume="295",
  number="-",
  pages="70--77",
  doi="10.1016/j.surfcoat.2015.10.076",
  issn="0257-8972",
  url="http://www.sciencedirect.com/science/article/pii/S0257897215303728"
}