Detail předmětu

Metrology and experimental data processing

FCH-DAO_METAk. rok: 2021/2022

Definition of metrology, its importance for environmental analysis. Development and optimization of analytical methods; repeatability of analyses.. Metrological characteristics of analytical methods; limit of detection, limit of quantification, return, traceability, robustness, control long-term stability analytical method validation and uncertainty. Standard operating procedure and quality assurance of analytical results.

Doporučená nebo povinná literatura

GRAFEN, A.. Modern statistics for the life sciences. Oxford: Oxford University Press, 2002. ISBN 01-992-5231-9. (EN)
MILLER, J. N. Statistics and chemometrics for analytical chemistry. 5th ed. Harlow: Pearson Prentice Hall, 2005, 268 s. ISBN 01-312-9192-0. (EN)
Ermer, J. and Miller, J. H. McB.: Method Validation in Pharmaceutical Analysis: a Guide to Best Prastice. Jonh Wiley & Sons, 2006, 418 s. ISBN: 978-3-527-31255-9. (EN)
HANRAHAN, G.: Environmental chemometrics: principles and modern application. CRC Press, 2009, 292 s. ISBN: 1420067966. (EN)
CHAN, Ch. Ch., LAM, H., ZHANG, X-M. Practical approaches to method validation and essential instrument qualification. Hoboken, N.J.: Wiley, c2010, xiv, 399 p. ISBN 04-701-2194-7. (EN)
BLIESNER, D. M. Validating chromatographic methods: a practical guide. Hoboken, N.J.: Wiley, c2006, viii, 291 p. ISBN 9780471741473. (EN)
REICHENBACHER, M. Challenges in analytical quality assurance. New York: Springer, 2011, p. cm. ISBN 978-3-642-16594-8. (EN)

Plánované vzdělávací činnosti a výukové metody

The course uses teaching methods in form of individual consultation. The e-learning system (LMS Moodle) is available to teachers and students.

Způsob a kritéria hodnocení

The knowledge will be tested by discussion - colloquium.

Jazyk výuky


Osnovy výuky

Definitions, nomenclature
Definition of basic and advanced parameteres, (yield, limits of detection, robustness of methods, linerarity, chemical parameters, uncertainty atd.)
General and advanced schemes of inorganic analyte determination in plants and tissues
Optimization and validation of analytical metods, regulations diagrams
Stability control in tarce analysis, yield determination, types of uncertainties


The aim is obtaining the knowledge on the metrology parameters related to trace analysis of componds and elements in various matrices.

Zařazení předmětu ve studijních plánech

  • Program DKAP_PCH doktorský, 1. ročník, zimní semestr, 0 kreditů, povinně volitelný
  • Program DPAP_FCH doktorský, 1. ročník, zimní semestr, 0 kreditů, povinně volitelný

Typ (způsob) výuky



20 hod., povinná

Vyučující / Lektor