Course detail

Analytical Methods of Technical Praxis

FCH-BCO_AMPAcad. year: 2018/2019

The course content is assembled from following thematic blocks: Basic rules of analytical sampling. Procedures for sample treatments: Isolation of target analytes from sample matrix (extraction by gal, liquid, solid phase), clean-up techniques (destructive, non-destructive), methods of group separation. Instrumental analytical methods used in analytical praxis: UV-VIS spectrophotometry, separation methods (gas and liquid chromatography, electromigration techniques), mass spectrometry. Basic principles of interpretation of analytical results. Uncertainty determination.

Learning outcomes of the course unit

Successful completion of the course will increase students knowledge, skills and competencies in the following areas:
1. Detailed knowledge of the complete analytical procedures used in routine analytical practice, consisting of an analytical sample collection, laboratory processing and the final analysis
2. Ability to design an optimal procedure for the analysis of a contaminant (or group of contaminants) in the environmental compartment, taking into account the specific conditions of the solved problem


Knowledge from courses Common and Inorganic Chemistry, Organic chemistry, Physical chemistry, Analytical chemistry I, Analytical Chemistry II


Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Klouda P.: Moderní analytické metody. 2. vyd. Ostrava: Nakladatelství Pavel Klouda, 2003 (CS)
Štulík, K. a. kol.: Analytické separační metody. 1. vyd. Praha: KU Praha, 2005 (CS)
Volka, K. a. kol.: Analytická chemie 2. 1. vyd. Praha: Vydavatelství VŠCHT, 1995 (CS)
E-learning na VUT: (CS)

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Student assessment takes the form of an oral examination in the range of themes discussed in the lectures. Assessed is the knowledge of the theoretical basis of the general analytical procedures and methods and student's ability of creative solution of discussed analytical problem.

Language of instruction


Work placements

Not applicable.

Course curriculum

1. Introduction, importance of analytical chemistry in technical praxis
2. Basic principles of analytical sampling
3. Methods of target analytes isolation from sample matrix
4. Techniques of clean-up and group separation
5. UV-VIS Spectrophotometry, principle and applications
6. Gas chromatography - principle, instrumentation, application in technical praxis
7. Liquid chromatography - principle, instrumentation, application in technical praxis
8. Electromigration methods - principle, classification, instrumentation, application in technical praxis
9. Mass spectrometry - physica basics, principle, instrumentation, acquiring and interpretation of mass spectra, application for the analysis of inorganic and organic materials
10. Hyphenated techniques and their application in technical praxis
11. Basic rules of analytical results treatment and interpretation


The aim of the course is to acquaint students with the methods and procedures used by the analytical practice of collection through his analytical sample processing to final analysis. They will also be familiarized with the methods and possibilities of mobile analytics and mobile analyzers usied for the control and protection of the environment, but also in the area of ​​security.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is recommended, but not checked.

Classification of course in study plans

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_CHTOZP , 3. year of study, winter semester, 4 credits, compulsory

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_CHTOZP , 3. year of study, winter semester, 4 credits, compulsory

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1. year of study, winter semester, 4 credits, compulsory

Type of course unit



26 hours, optionally

Teacher / Lecturer