Publication detail

Improvement of Electrophoresis Performance by Spectral Analysis

ABO KHAYAL, L. KIZEK, R. PROVAZNÍK, I.

Original Title

Improvement of Electrophoresis Performance by Spectral Analysis

Type

journal article - other

Language

English

Original Abstract

The paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using real-time spectral analysis of the samples to increase its performance. A laser beam illuminates the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.

Keywords

horizontal electrophoresis, laser, spectral analysis, agarose gel

Authors

ABO KHAYAL, L.; KIZEK, R.; PROVAZNÍK, I.

RIV year

2012

Released

12. 4. 2012

ISBN

1684-5315

Periodical

AFRICAN JOURNAL OF BIOTECHNOLOGY

Year of study

11

Number

51

State

Republic of Kenya

Pages from

11329

Pages to

11332

Pages count

4

BibTex

@article{BUT90646,
  author="Layal {Abo Khayal} and René {Kizek} and Valentine {Provazník}",
  title="Improvement of Electrophoresis Performance by Spectral Analysis",
  journal="AFRICAN JOURNAL OF BIOTECHNOLOGY",
  year="2012",
  volume="11",
  number="51",
  pages="11329--11332",
  issn="1684-5315"
}