Publication detail

Utilizing of square wave voltammetry to detect flavonoids in the presence of human urine

ADAM, V. MIKELOVÁ, R. HUBÁLEK, J. HANUSTIAK, P. BEKLOVÁ, M. HORNA, A. TRNKOVÁ, L. ZEMAN, L. KIZEK, R.

Original Title

Utilizing of square wave voltammetry to detect flavonoids in the presence of human urine

Type

journal article - other

Language

English

Original Abstract

About biological affecting of flavonoids on animal organisms is known less, thus we selected flavonoids, flavanones and flavones, and their glycosides, which were examined as potential inducers of cytochrome(s) P450 when administrated by gavages into experimental male rats. The study was focused on induction of CYP1A1, the major cytochrome P450 involved in carcinogen activation. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism (including colon microflora) in the body. After that we examined certain flavonoids as potential inducers of cytochrome P450, we wanted to suggest and optimize suitable electrochemical technique for determination of selected flavonoids (quercetin, quercitrin, rutin, chrysin and diosmin) in body liquids. For these purposes, we selected square wave voltannetry using carbon paste electrode.

Keywords

flavonoids; antioxidant; carbon paste electrode; square wave voltammetry; western blot analysis; cytochrome P450; cancer

Authors

ADAM, V.; MIKELOVÁ, R.; HUBÁLEK, J.; HANUSTIAK, P.; BEKLOVÁ, M.; HORNA, A.; TRNKOVÁ, L.; ZEMAN, L.; KIZEK, R.

Released

15. 10. 2007

Publisher

MDPI

Location

Basel, Švýcarsko

ISBN

1424-8220

Periodical

SENSORS

Year of study

7

Number

10

State

Swiss Confederation

Pages from

2402

Pages to

2418

Pages count

17

URL

http://www.mdpi.com/1424-8220/7/10/2402

Full text in the Digital Library

http://hdl.handle.net/11012/70075

BibTex

@article{BUT49680,
  author="ADAM, V. and MIKELOVÁ, R. and HUBÁLEK, J. and HANUSTIAK, P. and BEKLOVÁ, M. and HORNA, A. and TRNKOVÁ, L. and ZEMAN, L. and KIZEK, R.",
  title="Utilizing of square wave voltammetry to detect flavonoids in the presence of human urine",
  journal="SENSORS",
  year="2007",
  volume="7",
  number="10",
  pages="2402--2418",
  doi="10.3390/s7102402",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/7/10/2402"
}