Publication detail

Di-4-ANEPPS modulates electrical activity and progress of myocardial ischemia in rabbit isolated heart

RONZHINA, M. STRAČINA, T. LACINOVÁ, Ľ. ONDÁČOVÁ, K. PAVLOVIČOVÁ, M. MARŠÁNOVÁ, L. SMÍŠEK, R. JANOUŠEK, O. FIALOVÁ, K. KOLÁŘOVÁ, J. NOVÁKOVÁ, M. PROVAZNÍK, I.

Original Title

Di-4-ANEPPS modulates electrical activity and progress of myocardial ischemia in rabbit isolated heart

Type

journal article in Web of Science

Language

English

Original Abstract

Aims: Although voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart stained with di-4-ANEPPS is a suitable tool for myocardial ischemia investigation. Methods and Results: Study involved experiments on stained (n = 9) and non-stained (n = 11) Langendorff perfused rabbit isolated hearts. Electrophysiological effects of the dye were evaluated by analysis of various electrogram (EG) parameters using common paired and unpaired statistical tests. It was shown that staining the hearts with di-4-ANEPPS leads to only short-term sporadic prolongation of impulse conduction through atria and AV node. On the other hand, significant irreversible slowing of heart rate and ventricular conduction were found in stained hearts as compared to controls. In patch clamp experiments, significant inhibition of sodium current density was observed in differentiated NG108-15 cells stained by the dye. Although no significant differences in mean number of VPBs were found between the stained and the non-stained hearts in ischemia as well as in reperfusion, all abovementioned results indicate increased arrhythmogenicity. In isolated hearts during ischemia, prominent ischemic patterns appeared in the stained hearts with 3-4 min delay as compared to the non-stained ones. Moreover, the ischemic changes did not achieve the same magnitude as in controls even after 10 minutes of ischemia. It resulted in poor performance of ischemia detection by proposed EG parameters, as was quantified by receiver operating characteristics analysis. Conclusions: Our results demonstrate significant direct irreversible effect of di-4-ANEPPS on spontaneous heart rate and ventricular impulse conduction in rabbit isolated heart model. Particularly, this should be considered when di-4-ANEPPS is used in ischemia studies in rabbit. Delayed attenuated response of such hearts to ischemia might lead to misinterpretation of obtained results.

Keywords

Di-4-ANEPPS, Rabbit isolated heart, Myocardial Ischemia, Electrogram analysis, Patch- clamp, Voltage-sensitive dye

Authors

RONZHINA, M.; STRAČINA, T.; LACINOVÁ, Ľ.; ONDÁČOVÁ, K.; PAVLOVIČOVÁ, M.; MARŠÁNOVÁ, L.; SMÍŠEK, R.; JANOUŠEK, O.; FIALOVÁ, K.; KOLÁŘOVÁ, J.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

Released

10. 6. 2021

Publisher

Frontiers

ISBN

1664-042X

Periodical

Frontiers in Physiology

Year of study

12

Number

6

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

15

URL

https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full

Full text in the Digital Library

http://hdl.handle.net/11012/201544

BibTex

@article{BUT171652,
  author="Marina {Filipenská} and Tibor {Stračina} and Ľubica {Lacinová} and Katarína {Ondáčová} and Michaela {Pavlovičová} and Lucie {Šaclová} and Radovan {Smíšek} and Oto {Janoušek} and Kateřina {Fialová} and Jana {Kolářová} and Marie {Nováková} and Valentine {Provazník}",
  title="Di-4-ANEPPS modulates electrical activity and progress of myocardial ischemia in rabbit isolated heart",
  journal="Frontiers in Physiology",
  year="2021",
  volume="12",
  number="6",
  pages="1--15",
  doi="10.3389/fphys.2021.667065",
  issn="1664-042X",
  url="https://www.frontiersin.org/articles/10.3389/fphys.2021.667065/full"
}