Publication detail

Observation of lubrication mechanisms in knee replacement: A pilot study

NEČAS, D. SADECKÁ, K. VRBKA, M. GALLO, J. GALANDÁKOVÁ, A. KŘUPKA, I. HARTL, M.

Original Title

Observation of lubrication mechanisms in knee replacement: A pilot study

Type

journal article in Scopus

Language

English

Original Abstract

The present study introduces a unique experimental approach for in situ observation of lubricant film formation in knee joint replacements. A knee joint simulator was designed and equipped with optical module based on fluorescent optical method for film thickness observation. The contact between the femoral knee metal implant and real-shaped polymer insert mimicking actual contact nature is observed. The shape of the polymer insert was fabricated with respect to the shape of original polyethylene insert to ensure corresponding contact conformity. Simple solutions of albumin and γ-globulin proteins as well as its mixture were used while the film thickness was studied as a function of time considering simplified flexion/extension motion with variable load over the cycle. Adequate fluorescent markers were employed enabling to observe one particular protein during each measurement. The results showed a clear importance of the interaction of proteins since the mixtures showed different results compared to simple solutions. Especially considering albumin protein, its behaviour was substantially affected by adding γ-globulin. Moreover, a satisfactory compliance with previous findings related to hip joint lubrication in terms of the behaviour of both proteins was found. Finally, the motivation for future experimental work is highlighted.

Keywords

Knee replacement; lubrication; proteins; fluorescence

Authors

NEČAS, D.; SADECKÁ, K.; VRBKA, M.; GALLO, J.; GALANDÁKOVÁ, A.; KŘUPKA, I.; HARTL, M.

Released

1. 3. 2019

Publisher

Elsevier

Location

United Kingdom

ISBN

2352-5738

Periodical

Biotribology

Year of study

17

Number

2019

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

7

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S2352573818300416

BibTex

@article{BUT155469,
  author="NEČAS, D. and SADECKÁ, K. and VRBKA, M. and GALLO, J. and GALANDÁKOVÁ, A. and KŘUPKA, I. and HARTL, M.",
  title="Observation of lubrication mechanisms in knee replacement: A pilot study",
  journal="Biotribology",
  year="2019",
  volume="17",
  number="2019",
  pages="1--7",
  doi="10.1016/j.biotri.2019.02.001",
  issn="2352-5738",
  url="https://www.sciencedirect.com/science/article/pii/S2352573818300416"
}