Publication detail

Quantitative phase imaging in turbid media by coherence controlled holographic microscopy

CHMELÍK, R. ĎURIŠ, M. ŠTRBKOVÁ, L.

Original Title

Quantitative phase imaging in turbid media by coherence controlled holographic microscopy

English Title

Quantitative phase imaging in turbid media by coherence controlled holographic microscopy

Type

conference paper

Language

en

Original Abstract

Quantitative phase imaging (QPI) became an important technique for label-free biomedical imaging suitable particularly for observation of live cell and dry-mass profiling. Extension of this technique to objects immersed in turbid medium is highly desirable with respect to the need of non-invasive observation of live cells in real 3D environments. Coherence-controlled holographic microscopy is capable of QPI through turbid media owing to coherence gating induced in transmitted-light geometry by low spatial coherence of illumination. Using this approach, QPI of object in turbid medium can be formed both by ballistic and multiply scattered photons. Moreover, the particular QPIs formed by ballistic and scattered photons can be superimposed thus yielding synthetic QPI of substantially improved image quality. We support the theoretical reasoning of the effect by experimental data.

English abstract

Quantitative phase imaging (QPI) became an important technique for label-free biomedical imaging suitable particularly for observation of live cell and dry-mass profiling. Extension of this technique to objects immersed in turbid medium is highly desirable with respect to the need of non-invasive observation of live cells in real 3D environments. Coherence-controlled holographic microscopy is capable of QPI through turbid media owing to coherence gating induced in transmitted-light geometry by low spatial coherence of illumination. Using this approach, QPI of object in turbid medium can be formed both by ballistic and multiply scattered photons. Moreover, the particular QPIs formed by ballistic and scattered photons can be superimposed thus yielding synthetic QPI of substantially improved image quality. We support the theoretical reasoning of the effect by experimental data.

Keywords

quantitative phase imaging, turbid-media imaging, holographic microscopy, coherence gating

Released

24.05.2018

Publisher

Society of Photo-Optical Instrumentation Engineers (SPIE)

Location

Strasbourg, France

ISBN

0277-786X

Periodical

Proceedings of SPIE

Year of study

106771C

State

US

Pages from

1

Pages to

12

Pages count

12

URL

Documents

BibTex


@inproceedings{BUT147735,
  author="Radim {Chmelík} and Miroslav {Ďuriš} and Lenka {Štrbková}",
  title="Quantitative phase imaging in turbid
media by coherence controlled
holographic microscopy",
  annote="Quantitative phase imaging (QPI) became an important technique for label-free biomedical imaging suitable particularly for observation of live cell and dry-mass profiling. Extension of this technique to objects immersed in turbid medium is highly desirable with respect to the need of non-invasive observation of live cells in real 3D environments. Coherence-controlled holographic microscopy is capable of QPI through turbid media owing to coherence gating induced in transmitted-light geometry by low spatial coherence of illumination. Using this approach, QPI of object in turbid medium can be formed both by ballistic and multiply scattered photons. Moreover, the particular QPIs formed by ballistic and scattered photons can be superimposed thus yielding synthetic QPI of substantially improved image quality. We support the theoretical reasoning of the effect by experimental data.",
  address="Society of Photo-Optical Instrumentation Engineers (SPIE)",
  booktitle="Unconventional Optical Imaging",
  chapter="147735",
  doi="10.1117/12.2309907",
  howpublished="online",
  institution="Society of Photo-Optical Instrumentation Engineers (SPIE)",
  year="2018",
  month="may",
  pages="1--12",
  publisher="Society of Photo-Optical Instrumentation Engineers (SPIE)",
  type="conference paper"
}