Publication detail

Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes

PUTEROVÁ, J. RAZUMOVA, O. MARTÍNEK, T. ALEXANDROV, O. DIVASHUK, M. KUBÁT, Z. HOBZA, R. KARLOV, G. KEJNOVSKÝ, E.

Original Title

Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes

Type

journal article in Web of Science

Language

English

Original Abstract

Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.

Keywords

sex chromosomes, genome composition, chromosomal localization, repetitive DNA

Authors

PUTEROVÁ, J.; RAZUMOVA, O.; MARTÍNEK, T.; ALEXANDROV, O.; DIVASHUK, M.; KUBÁT, Z.; HOBZA, R.; KARLOV, G.; KEJNOVSKÝ, E.

Released

2. 2. 2017

ISBN

1759-6653

Periodical

GENOME BIOL EVOL

Year of study

9

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

197

Pages to

212

Pages count

16

URL

https://academic.oup.com/gbe/article/9/1/197/2830930

Full text in the Digital Library

http://hdl.handle.net/11012/137198

BibTex

@article{BUT144426,
  author="Janka {Puterová} and Olga {Razumova} and Tomáš {Martínek} and Oleg {Alexandrov} and Mikhail {Divashuk} and Zdeněk {Kubát} and Roman {Hobza} and Gennady {Karlov} and Eduard {Kejnovský}",
  title="Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes",
  journal="GENOME BIOL EVOL",
  year="2017",
  volume="9",
  number="1",
  pages="197--212",
  doi="10.1093/gbe/evw303",
  issn="1759-6653",
  url="https://academic.oup.com/gbe/article/9/1/197/2830930"
}