Publication detail

Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel

HOLÍNKOVÁ, P. PEKAŘ, M.

Original Title

Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel

Type

abstract

Language

English

Original Abstract

This fluorescence study deals with the microstructural changes of physical hydrogel formed by mixing hyaluronan solution with oppositely-charged surfactant cetyltrimethylammonium bromide (CTAB), where micelles of CTAB form directly either physical crosslinks of the hyaluronan hydrogel network. Fluorescence measurements were carried out on this hyaluronan-based hydrogels containing two different fluorescence probes, hydrophobic perylene and hydrophilic one, rhodamine 6G. The excitation and emission spectra, fluorescence intensity, steady-state fluorescence anisotropy and lifetime of probes in the samples were measured. The fluorescence anisotropy values obtained in these experiments were used to estimate a microviscosity of the corresponding hydrogel regions during ageing. It is observed that the hydrophobic domains do not undergo significant structural changes. However, the microviscosity of hydrophilic parts gradually decreases, which indicates some structural changes in the nearby area of rhodamine molecules during ageing.

Keywords

hydrogel, hyaluronan, CTAB, microviscosity, fluorescence anisotropy

Authors

HOLÍNKOVÁ, P.; PEKAŘ, M.

Released

11. 9. 2016

Publisher

Leibniz-Institut für Polymerforschung Dresden e. V.

Location

Dresden, Germany

ISBN

978-3-9816007-4-2

Book

20th European Symposium on Polymer Spectroscopy ESOPS 20

Pages from

65

Pages to

65

Pages count

1

BibTex

@misc{BUT128284,
  author="Petra {Holínková} and Miloslav {Pekař}",
  title="Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel",
  booktitle="20th European Symposium on Polymer Spectroscopy ESOPS 20",
  year="2016",
  pages="65--65",
  publisher="Leibniz-Institut für Polymerforschung Dresden e. V.",
  address="Dresden, Germany",
  isbn="978-3-9816007-4-2",
  note="abstract"
}