Publication detail

Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

ŠTRBKOVÁ, L. CHMELÍK, R. VESELÝ, P.

Original Title

Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

English Title

Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy

Type

conference proceedings

Language

en

Original Abstract

The understanding of the surface-cell interaction plays important role for the biomaterials development and bioengineering. We present the Coherence-Controlled Holographic Microscope (CCHM) built in Brno University of Technology as a tool for a reliable assessment of the biocompatibility of the amine-rich coatings. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of the amine layers properties on cell adhesion need to be further investigated. Imaging and assessment of surface-cell interaction is an arduous task, since the cells are weakly scattering and absorbing specimens. The CCHM enables to acquire speckle-free optically-sectioned quantitative phase images of live cells in high contrast without using any labels. The phase image contains quantitative information, from which valuable parameters directly related to the cell mass can be obtained. Based on those parameters, the biocompatibility of the surfaces is evaluated. The stable amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency capacitively coupled discharge. The normal human dermal fibroblasts were plated on sterilized plasma treated coverslips and cultivated for 2 days. The cell-surface interaction was imaged by the Coherence-Controlled Holographic Microscope and biocompatibility was assessed. Results show that amine-rich films proved to act as biocompatible surfaces that enhanced cells adhesion and proliferation.

English abstract

The understanding of the surface-cell interaction plays important role for the biomaterials development and bioengineering. We present the Coherence-Controlled Holographic Microscope (CCHM) built in Brno University of Technology as a tool for a reliable assessment of the biocompatibility of the amine-rich coatings. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of the amine layers properties on cell adhesion need to be further investigated. Imaging and assessment of surface-cell interaction is an arduous task, since the cells are weakly scattering and absorbing specimens. The CCHM enables to acquire speckle-free optically-sectioned quantitative phase images of live cells in high contrast without using any labels. The phase image contains quantitative information, from which valuable parameters directly related to the cell mass can be obtained. Based on those parameters, the biocompatibility of the surfaces is evaluated. The stable amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency capacitively coupled discharge. The normal human dermal fibroblasts were plated on sterilized plasma treated coverslips and cultivated for 2 days. The cell-surface interaction was imaged by the Coherence-Controlled Holographic Microscope and biocompatibility was assessed. Results show that amine-rich films proved to act as biocompatible surfaces that enhanced cells adhesion and proliferation.

Keywords

quantitative phase imaging, Coherence-Controlled Holographic Microscope, cyclopropylamine, amine-rich coating

Released

27.03.2015

Location

Göttingen, Germany

ISBN

978-3-95404-942

Book

Focus on microscopy 2015

Pages count

1

URL

Documents

BibTex


@proceedings{BUT114392,
  author="Lenka {Štrbková} and Radim {Chmelík} and Pavel {Veselý}",
  title="Biocompatibility assessment of cyclopropylamine plasma polymers studied by coherence-controlled holographic microscopy",
  annote="The understanding of the surface-cell interaction plays important role for the biomaterials
development and bioengineering. We present the Coherence-Controlled Holographic
Microscope (CCHM) built in Brno University of Technology as a tool for a reliable
assessment of the biocompatibility of the amine-rich coatings.
Although it is already known that amine groups increase the cell adhesion and proliferation,
the influence of the amine layers properties on cell adhesion need to be further investigated.
Imaging and assessment of surface-cell interaction is an arduous task, since the cells are
weakly scattering and absorbing specimens. The CCHM enables to acquire speckle-free
optically-sectioned quantitative phase images of live cells in high contrast without using any
labels. The phase image contains quantitative information, from which valuable
parameters directly related to the cell mass can be obtained. Based on those parameters, the
biocompatibility of the surfaces is evaluated.
The stable amine-rich coatings were prepared by low pressure plasma polymerization of
cyclopropylamine using radio frequency capacitively coupled discharge. The normal human
dermal fibroblasts were plated on sterilized plasma treated coverslips and cultivated for
2 days. The cell-surface interaction was imaged by the Coherence-Controlled Holographic
Microscope and biocompatibility was assessed. Results show that amine-rich films proved to
act as biocompatible surfaces that enhanced cells adhesion and proliferation.",
  booktitle="Focus on microscopy 2015",
  chapter="114392",
  howpublished="print",
  year="2015",
  month="march",
  type="conference proceedings"
}