Publication detail

Dynamic phase differences method for the assessment of cellular dynamic processes

ŠTRBKOVÁ, L. KŘÍŽOVÁ, A. ČOLLÁKOVÁ, J. CHMELÍK, R. VESELÝ, P.

Original Title

Dynamic phase differences method for the assessment of cellular dynamic processes

English Title

Dynamic phase differences method for the assessment of cellular dynamic processes

Type

conference proceedings

Language

en

Original Abstract

Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploits the quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).

English abstract

Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploits the quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).

Keywords

quantitative phase imaging, holographic microscopy

Released

21.10.2014

Publisher

Muni PRESS

Location

Brno

ISBN

978-80-210-7159-9

Book

Book of Abstracts CEITEC Annual Conference

Pages from

109

Pages to

109

Pages count

1

URL

Documents

BibTex


@proceedings{BUT114385,
  author="Lenka {Štrbková} and Aneta {Křížová} and Jana {Čolláková} and Radim {Chmelík} and Pavel {Veselý}",
  title="Dynamic phase differences method for the assessment of cellular dynamic processes",
  annote="Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploits the quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).",
  address="Muni PRESS",
  booktitle="Book of Abstracts CEITEC Annual Conference",
  chapter="114385",
  howpublished="online",
  institution="Muni PRESS",
  year="2014",
  month="october",
  pages="109--109",
  publisher="Muni PRESS",
  type="conference proceedings"
}