Publication detail

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

KREMPLOVÁ, M. FIALOVÁ, D. NEJDL, L. HYNEK, D. TRNKOVÁ, L. HUBÁLEK, J. KIZEK, R. ADAM, V.

Original Title

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Type

journal article in Web of Science

Language

English

Original Abstract

The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detecte - peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.

Keywords

adenine; adenine interaction; magnetic beads; square wave voltammetry; aptamer; biosensor; nanobiotechnology

Authors

KREMPLOVÁ, M.; FIALOVÁ, D.; NEJDL, L.; HYNEK, D.; TRNKOVÁ, L.; HUBÁLEK, J.; KIZEK, R.; ADAM, V.

RIV year

2014

Released

1. 3. 2014

Publisher

MDPI

ISBN

1996-1944

Periodical

Materials

Year of study

7

Number

3

State

Swiss Confederation

Pages from

1455

Pages to

1472

Pages count

18

URL

http://www.mdpi.com/1996-1944/7/3/1455

Full text in the Digital Library

http://hdl.handle.net/11012/63708

BibTex

@article{BUT111195,
  author="Monika {Kremplová} and Dana {Fialová} and Lukáš {Nejdl} and David {Hynek} and Libuše {Trnková} and Jaromír {Hubálek} and René {Kizek} and Vojtěch {Adam}",
  title="Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure",
  journal="Materials",
  year="2014",
  volume="7",
  number="3",
  pages="1455--1472",
  doi="10.3390/ma7031455",
  issn="1996-1944",
  url="http://www.mdpi.com/1996-1944/7/3/1455"
}