Publication detail

Automatized determination of metallothionein by adsorptive transfer stripping technique coupled with Brdicka reaction

HYNEK, D. TMEJOVÁ, K. TRNKOVÁ, L. HUBÁLEK, J. ADAM, V. KIZEK, R.

Original Title

Automatized determination of metallothionein by adsorptive transfer stripping technique coupled with Brdicka reaction

Type

book chapter

Language

English

Original Abstract

Application of electrochemical methods for protein determination was discovered in the thirties of the last century. Rudolf Brdicka was one who focused his attention to the protein electrochemical determination and used discovered analytical method for clinical purposes. Brdicka way of protein detection was based on the specific reaction between thiol group of proteins with Brdicka solution. Metallothionein (MT) as a low-molecular mass protein with high thiols content seems to be ideal target for such detection. This protein is involved in heavy metals homeostasis due to their chelation centers in MT structure.

Keywords

metallothionein, Brdicka reaction, thiol compound, mercury electrode, tumour diseases, automation

Authors

HYNEK, D.; TMEJOVÁ, K.; TRNKOVÁ, L.; HUBÁLEK, J.; ADAM, V.; KIZEK, R.

RIV year

2013

Released

1. 6. 2013

Publisher

Nova Science Publisher, Inc.

ISBN

978-1-62948-057-2

Book

Voltammetry: Theory, Types and Applications

Edition number

1

Pages from

1

Pages to

12

Pages count

12

URL

http://web2.mendelu.cz/af_239_nanotech/data/pub/Kizek%20from%20978-1-62948-057-2.pdf

BibTex

@inbook{BUT101248,
  author="David {Hynek} and Kateřina {Tmejová} and Libuše {Trnková} and Jaromír {Hubálek} and Vojtěch {Adam} and René {Kizek}",
  title="Automatized determination of metallothionein by adsorptive transfer stripping technique coupled with Brdicka reaction",
  booktitle="Voltammetry: Theory, Types and Applications",
  year="2013",
  publisher="Nova Science Publisher, Inc.",
  edition="1",
  pages="1--12",
  isbn="978-1-62948-057-2",
  url="http://web2.mendelu.cz/af_239_nanotech/data/pub/Kizek%20from%20978-1-62948-057-2.pdf"
}