Publication result detail

Holographic Incoherent-light-source Quantitative Phase Imaging: An Alternative to Holographic Tomography

CHMELIK, R.; MICHALKOVA, I.; DOSTAL, Z.; DURIS, M.; SLABA, M.

Original Title

Holographic Incoherent-light-source Quantitative Phase Imaging: An Alternative to Holographic Tomography

English Title

Holographic Incoherent-light-source Quantitative Phase Imaging: An Alternative to Holographic Tomography

Type

Paper in proceedings (conference paper)

Original Abstract

The Holographic Incoherent-light-source Quantitative Phase Imaging (hiQPI) technique provides optical sectioning capability similar to confocal microscopy due to the coherence-gating effect. Using this capability for 3D imaging, hiQPI becomes a full-fledged alternative to Holographic Tomography (HT). We used the first Born approximation of scattering theory to compare the spatial-frequency transfer properties of the two 3D imaging techniques for weakly scattering specimens. We derived the 3D coherent transfer functions, which turned out to be identical for HT and hiQPI approaches. The reconstruction of the 3D refractive index distribution of the phantom from simulated hiQPI and HT data confirmed this theoretical prediction, while the reconstruction performed on experimental red blood cell data verified the feasibility of the hiQPI approach.

English abstract

The Holographic Incoherent-light-source Quantitative Phase Imaging (hiQPI) technique provides optical sectioning capability similar to confocal microscopy due to the coherence-gating effect. Using this capability for 3D imaging, hiQPI becomes a full-fledged alternative to Holographic Tomography (HT). We used the first Born approximation of scattering theory to compare the spatial-frequency transfer properties of the two 3D imaging techniques for weakly scattering specimens. We derived the 3D coherent transfer functions, which turned out to be identical for HT and hiQPI approaches. The reconstruction of the 3D refractive index distribution of the phantom from simulated hiQPI and HT data confirmed this theoretical prediction, while the reconstruction performed on experimental red blood cell data verified the feasibility of the hiQPI approach.

Keywords

3D quantitative phase imaging | cancer research | coherence gating | low-coherence effects | off-axis digital holography | personalized treatment

Key words in English

3D quantitative phase imaging | cancer research | coherence gating | low-coherence effects | off-axis digital holography | personalized treatment

Authors

CHMELIK, R.; MICHALKOVA, I.; DOSTAL, Z.; DURIS, M.; SLABA, M.

RIV year

2026

Released

01.01.2025

Publisher

SPIE

ISBN

9781510684065

Book

Progress in Biomedical Optics and Imaging Proceedings of SPIE

Periodical

Proceedings of SPIE

State

United States of America

Pages count

6

URL

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/13329/3039015/Holographic-incoherent-light-source-quantitative-phase-imaging--an-alternative/10.1117/12.3039015.short

BibTex

@inproceedings{BUT199606,
  author="Radim {Chmelík} and Ivana {Michálková} and Zbyněk {Dostál} and Miroslav {Ďuriš} and Michala {Slabá}",
  title="Holographic Incoherent-light-source Quantitative Phase Imaging: An Alternative to Holographic Tomography",
  booktitle="Progress in Biomedical Optics and Imaging Proceedings of SPIE",
  year="2025",
  journal="Proceedings of SPIE",
  pages="6",
  publisher="SPIE",
  doi="10.1117/12.3039015",
  isbn="9781510684065",
  issn="0277-786X",
  url="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/13329/3039015/Holographic-incoherent-light-source-quantitative-phase-imaging--an-alternative/10.1117/12.3039015.short"
}