Publication result detail

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

CERNEI, N.; KOPEL, P.; HORKÝ, P.; HYNEK, D.; ZÍTKA, O.; ADAM, V.

Original Title

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

English Title

Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles

Type

Paper in proceedings (conference paper)

Original Abstract

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (γ-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.

English abstract

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) - leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (γ-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.

Keywords

Ion Exchange Chromatography; Maghemite; MWCNT; BCAAs; valine; leucine; arginine

Key words in English

Ion Exchange Chromatography; Maghemite; MWCNT; BCAAs; valine; leucine; arginine

Authors

CERNEI, N.; KOPEL, P.; HORKÝ, P.; HYNEK, D.; ZÍTKA, O.; ADAM, V.

RIV year

2021

Released

10.06.2020

Publisher

Tanger

ISBN

978-80-87294-95-6

Book

NANOCON 2019 CONFERENCE PROCEEDINGS

Edition

1.

Pages from

374

Pages to

378

Pages count

5

URL

https://doi.org/10.37904/nanocon.2019.8497

Full text in the Digital Library

http://hdl.handle.net/11012/196672

BibTex

@inproceedings{BUT164259,
  author="Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Pavel {Horký} and David {Hynek} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Isolation of branched chain aminoacids (Valine, Leucine and Arginine) using maghemite particles",
  booktitle="NANOCON 2019 CONFERENCE PROCEEDINGS",
  year="2020",
  series="1.",
  pages="374--378",
  publisher="Tanger",
  doi="10.37904/nanocon.2019.8497",
  isbn="978-80-87294-95-6",
  url="https://doi.org/10.37904/nanocon.2019.8497"
}

Documents

nanocon2019_cernei_nanocon